Designed for improved chromatography, Thermo Scientific™ Hypersil GOLD™ columns are the culmination of over 35 years of experience in the product development and manufacturing of HPLC media and columns.
The range and capabilities of this state-of-the-art family of columns, with numerous chemistries and a range of particle sizes and hardware formats meet the challenges of modern chromatography.
The highly pure Hypersil GOLD silica is manufactured, bonded and packed inISO 9001:2008 accredited facilities, operating under strict protocols using robust procedures and extensive quality control testing. The manufacturing and bondingprocess creates an even surface with very few silanols leading to reduced secondary interactions. This ensures consistent performance, column after column.
Before starting ensure that no buffers or samples are present on the column and that the mobile phase used prior to cleaning is miscible with the first wash solvent.
Using 50% of the working flow rate, flush the column with 40 to 60 column volumes of each of the solvents below in the order shown.
After cleaning, ensure that the mobile phase is miscible with the last solvent in the column.
Hypersil GOLD HPLC columns are available in 12 different chemistries to optimize separations and maximize productivity. The extensive range of Hypersil GOLD columns offers chromatographers outstanding peak shape for reversed phase, ion exchange, HILIC or normal phase chromatography. With all 12 phases being available with 1.9 μm particle size, Hypersil GOLD columns offer chromatographers flexibility in choosing the correct column, whether they are using conventional or ultra-high pressure LC systems.